賽維爾生物服務、產品資料庫

賽維爾生物

TEL:400-6027-178

賽維爾生物

一抗

  1. 首頁
  2. 全部分類
  3. 產品
  4. 抗體
  5. 一抗
  6. 內容

Anti -Histone H2A.X Rabbit pAb

貨號:GB111560

品牌:Servicebio

價格:¥650

規格: 100 μl

下載說明書
附件下載地址: ×
/e/DownSys/DownSoft/?classid=18&id=4244&pathid=0

Product Information

 

Description Histone H2A.X rabbit polyclonal
Protein full name Histone H2AX
Synonyms H2a/x, H2AFX, H2AX
Immunogen KLH conjugated Synthetic peptide
corresponding to Human Histone H2A.X
Isotype IgG
Purity Affinity purification
Subcellular location Nucleus
Predicted MW. 15 kDa
Observed MW. 15 kDa
Uniprot ID P16104, P27661

 


 

Applications

 

applications species dilution Positive tissue
WB Human,
Mouse, Rat
1: 500-1: 1000 testis, kidney, thymus
IHC Mouse, Rat 1: 1000-1: 2000 testis, kidney
IF Mouse, Rat 1: 100-1: 300 kidney, testis

 


 

Background
 

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

 


 

Images

 


Western blot analysis of H2AX (GB111560) 
at dilution of 1: 1000
Lane 1: HepG2 cell lysate
Lane 2: A549 cell lysate
Lane 3: Hela cell lysate
Lane 4: Mouse testis tissue lysate
Lane 5: Mouse kidney tissue lysate
Lane 6: Mouse thymus tissue lysate
Lane 7: Rat testis tissue lysate
Lane 8: Rat kidney tissue lysate
Lane 9: Rat thymus tissue lysate
 

Immunohistochemistry analysis of paraffin-embedded mouse testis using H2AX (GB111560) at dilution of 1: 2000
 

Immunohistochemistry analysis of paraffin-embedded mouse testis using H2AX (GB111560) at dilution of 1: 2000
 

Immunohistochemistry analysis of paraffin-embedded mouse kidney using H2AX (GB111560) at dilution of 1: 2000
 

Immunohistochemistry analysis of paraffin-embedded rat kidney using H2AX (GB111560) at dilution of 1: 2000
 

Immunofluorescent analysis of paraformaldehyde-fixed mouse kidney using H2AX (GB111560) at dilution of 1: 300
 

Immunofluorescent analysis of paraformaldehyde-fixed rat kidney using H2AX (GB111560) at dilution of 1: 300
 

Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using H2AX (GB111560) at dilution of 1: 300
 

Immunofluorescent analysis of paraformaldehyde-fixed rat testis using H2AX (GB111560) at dilution of 1: 300
 

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.
 

 

NOTE: This product is intended for research only.

 

11选5走势图北京